Simple colorimetric method for detecting degenerate strains of the cultivated basidiomycete Flammulina velutipes (Enokitake).
نویسندگان
چکیده
Degeneration of cultivated strains of Flammulina velutipes is a serious problem. We developed a simple colorimetric method to detect degenerate strains by using a liquid medium supplemented with bromothymol blue and lactose. The ability of a strain to develop normal mushrooms could be determined by the color of the medium.
منابع مشابه
Draft Genome Sequence of the Basidiomycetous Fungus Flammulina velutipes TR19
Here, we report the draft genome sequence of Flammulina velutipes TR19, which was newly isolated from commercial strains in Japan. The genes related to fruiting body formation in the basidiomycete were identified by whole-genome analysis.
متن کاملIdentification of Degenerate Nuclei and Development of a SCAR Marker for Flammulina velutipes
Flammulina velutipes is one of the major edible mushrooms in the world. Recently, abnormalities that have a negative impact on crop production have been reported in this mushroom. These symptoms include slow vegetative growth, a compact mycelial mat, and few or even no fruiting bodies. The morphologies and fruiting capabilities of monokaryons of wild-type and degenerate strains that arose throu...
متن کاملFruiting at High Temperature and Its Genetic Control in the Basidiomycete Flammulina velutipes.
Of 10 geographic strains of Flammulina velutipes, 4 were found capable of fruiting at 22 degrees C (Fr(H)) rather than at the typical 15 degrees C (Fr(L)). Crosses made between Fr(H) and Fr(L) monokaryons were never observed to fruit at 22 degrees C. However, some hybrids did fruit at the intermediate temperature of 18 degrees C when grown on appropriate substrates, indicating incomplete domina...
متن کاملCloning and sequencing analysis of Trp1 gene of Flammulina velutipes.
The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-...
متن کاملHighly-efficient liposome-mediated transformation system for the basidiomycetous fungus Flammulina velutipes.
Flammulina velutipes is a well-known edible mushroom cultivated all over the world. However, because of the low transformation frequency, the expensive instruments required, and the complicated, time-consuming procedures necessary, there is insufficient genetic research on F. velutipes. In this study, we report a liposome-mediated transformation (LMT) system for the genetic transformation of F....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 10 شماره
صفحات -
تاریخ انتشار 2005